Gabriel M K, McGuinness E T
FEBS Lett. 1984 Oct 1;175(2):419-21. doi: 10.1016/0014-5793(84)80780-2.
Low-angle laser-light scattering (LALLS) was employed to measure the absolute molecular weight of chicken liver NAD+ kinase (NADK). The weight-average molecular weight (Mw) was found to be 275 000 +/- 15 000. The corresponding value for the second virial coefficient was -1.65 X 10(-3) ml X mol X g2. The value for Mw is in close accord with estimates reported for pigeon liver (270 000) and C. utilis (260 000) NADK. If the active enzyme is a dimer, the weight difference between pigeon/chicken liver and rabbit liver (136 000) NADK would indicate that the latter enzyme is an active monomer unit.
采用低角度激光光散射（LALLS）法测定鸡肝NAD⁺激酶（NADK）的绝对分子量。测得的重均分子量（Mw）为275 000 ± 15 000。第二维里系数的相应值为-1.65×10⁻³ ml·mol·g²。Mw值与报道的鸽肝（270 000）和产朊假丝酵母（260 000）NADK的估计值非常一致。如果活性酶是二聚体，鸽/鸡肝与兔肝（136 000）NADK之间的重量差异表明后者的酶是活性单体单元。
