猪蛔虫中的多聚（ADP-核糖基化）

Poly(ADP-ribosylation) in Ascaris suum.

作者信息

Walter R D, Ossikovski E

出版信息

Hoppe Seylers Z Physiol Chem. 1984 Jul;365(7):805-8. doi: 10.1515/bchm2.1984.365.2.805.

DOI:10.1515/bchm2.1984.365.2.805

Abstract

Poly(ADP-ribosylation) was demonstrated in the intestinal parasite Ascaris suum, especially in the reproductive tissues. The activity of the ADP-ribosyltransferase was found to depend on divalent cations and to be stimulated by deoxyribonuclease I about 5-fold. The reaction rate was optimal at a temperature of 30 degrees C and at pH about 8.4. The apparent Km value for NAD was estimated to be 0.2mM. The enzyme activity was effectively inhibited by nicotinamide (Ki = 65 microM) benzamide (6 microM), 3-aminobenzamide (10 microM), theophylline (35 microM) and thymidine (50 microM). The type of inhibition by these compounds was found to be competitive with respect to NAD.

摘要

在肠道寄生虫猪蛔虫中发现了多聚（ADP-核糖基化）现象，尤其是在生殖组织中。已发现ADP-核糖基转移酶的活性依赖于二价阳离子，并受到脱氧核糖核酸酶I的刺激，刺激倍数约为5倍。反应速率在30摄氏度的温度和约8.4的pH值下最佳。NAD的表观Km值估计为0.2mM。该酶活性受到烟酰胺（Ki = 65 microM）、苯甲酰胺（6 microM）、3-氨基苯甲酰胺（10 microM）、茶碱（35 microM）和胸苷（50 microM）的有效抑制。发现这些化合物的抑制类型相对于NAD是竞争性的。