质粒pSC101分配位点的体外缺失
In vitro deletions in the partition locus of plasmid pSC101.
作者信息
Kalla S R, Gustafsson P
出版信息
J Bacteriol. 1984 Oct;160(1):434-7. doi: 10.1128/jb.160.1.434-437.1984.
Abstract
Deletion mutants in the 375-base-pair EcoRI-AvaI fragment carrying the partition locus of plasmid pSC101 were formed by the combined action of exonuclease III and nuclease S1. Six deletion mutants were isolated, and the endpoints of the deletions were sequenced. One of the deletions extended 69 base pairs from the EcoRI site without impairing plasmid stability. The other five deletions caused the plasmid to be unstable and extended 199 to 251 base pairs from the EcoRI site.
摘要
携带质粒pSC101分配位点的375个碱基对的EcoRI-AvaI片段中的缺失突变体是由核酸外切酶III和核酸酶S1的联合作用形成的。分离出六个缺失突变体，并对缺失的端点进行了测序。其中一个缺失从EcoRI位点延伸了69个碱基对，而不损害质粒稳定性。其他五个缺失导致质粒不稳定，并从EcoRI位点延伸了199至251个碱基对。
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