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源自质粒pSC101的质粒pMB9上启动子的结构。

Structure of a promotor on plasmid pMB9 derived from plasmid pSC101.

作者信息

Pannekoek H, Maat J, van den Berg E, Noordermeer I

出版信息

Nucleic Acids Res. 1980 Apr 11;8(7):1535-50. doi: 10.1093/nar/8.7.1535.

DOI:10.1093/nar/8.7.1535
PMID:6253940
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC324015/
Abstract

The DNA sequence of a 354 basepair EcoRI-HindIII fragment of plasmid pMB9 which has originally been derived from plasmid pSC101 has been resolved. This fragment contains a promoter for transcription directed towards the EcoRI site. Escherichia coli RNA polymerase binds to a region within the EcoRI-HindIII fragment which contains the heptamer 5' TATGGTG (132-126) and the duodecamer 5' TGATGAACATCA (158-147). Based on commonalities with other promotors these DNA sequences probably function as, respectively, "binding site" and "recognition site". Furthermore, this fragment harbours a translation reading frame free of nonsense codons and at about 25 basepairs from the indicated heptamer a nucleotide sequence which meets with the requirements for initiation of translation. By heteroduplex mapping it was shown that the EcoRI-HindIII fragment has been derived from a region near or within the origin of replication of pSC101. The copynumber of plasmids containing the EcoRI-HindIII fragment is two-fold lower than that of plasmids lacking this fragment. This effect might be related to the original function of this fragment on plasmid pSC101.

摘要

最初源自质粒pSC101的质粒pMB9的一个354碱基对的EcoRI - HindIII片段的DNA序列已被解析。该片段包含一个朝向EcoRI位点的转录启动子。大肠杆菌RNA聚合酶结合到EcoRI - HindIII片段内的一个区域，该区域包含七聚体5'TATGGTG（132 - 126）和十二聚体5'TGATGAACATCA（158 - 147）。基于与其他启动子的共性，这些DNA序列可能分别作为“结合位点”和“识别位点”发挥作用。此外，该片段含有一个无无义密码子的翻译阅读框，并且在距所示七聚体约25个碱基对处有一个符合翻译起始要求的核苷酸序列。通过异源双链体图谱分析表明，EcoRI - HindIII片段源自pSC101复制起点附近或内部的一个区域。含有EcoRI - HindIII片段的质粒的拷贝数比缺乏该片段的质粒低两倍。这种效应可能与该片段在质粒pSC101上的原始功能有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/367a/324015/014c16422119/nar00424-0108-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/367a/324015/b70e05e54f0c/nar00424-0100-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/367a/324015/13ba34134fe7/nar00424-0103-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/367a/324015/014c16422119/nar00424-0108-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/367a/324015/b70e05e54f0c/nar00424-0100-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/367a/324015/13ba34134fe7/nar00424-0103-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/367a/324015/014c16422119/nar00424-0108-a.jpg

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