Schwartz A L, Steer C J, Kempner E S
J Biol Chem. 1984 Oct 10;259(19):12025-9.
The radiation target size of the functional asialoglycoprotein receptor (ASGP-R) in purified plasma membranes was determined for human liver and the human hepatoma cell line, Hep G2, by evaluation of the binding of 125I-asialoorosomucoid. Identical inactivation curves were observed for ASGP-R from normal human liver and post-mortem human liver and exhibited a functional unit of 70,000 daltons. The human hepatoma Hep G2 ASGP-R, in contrast, showed a target size of 140,000 daltons. These results suggest that the functional ligand-binding unit of the human ASGP-R is a multimer of the approximately 34,000-dalton unglycosylated receptor polypeptide (Schwartz, A. L., and Rup, D. (1983) J. Biol. Chem. 258, 11249-11255).
通过评估¹²⁵I-去唾液酸糖蛋白的结合情况，测定了人肝脏和人肝癌细胞系Hep G2纯化质膜中功能性去唾液酸糖蛋白受体（ASGP-R）的辐射靶标大小。观察到正常人肝脏和尸检人肝脏的ASGP-R具有相同的失活曲线，且表现出一个70,000道尔顿的功能单位。相比之下，人肝癌Hep G2的ASGP-R显示出140,000道尔顿的靶标大小。这些结果表明，人ASGP-R的功能性配体结合单位是约34,000道尔顿未糖基化受体多肽的多聚体（施瓦茨，A.L.，和鲁普，D.（1983年）《生物化学杂志》258, 11249 - 11255）。
