单克隆抗体LICR-LON-E36可识别雌性大鼠垂体中的促性腺激素细胞。
Monoclonal antibody LICR-LON-E36 recognizes gonadotrophs in female rat pituitaries.
作者信息
Monaghan P, Sappino A P, Roberts J D, Knight M A, Ellis J, Nakajima T
出版信息
J Histochem Cytochem. 1984 Oct;32(10):1048-54. doi: 10.1177/32.10.6090532.
The distribution of the antigen localized by monoclonal antibody LICR-LON-E36 has been studied by means of light and electron microscopical immunocytochemical procedures on resin-embedded pituitaries from male and female rats. Using both immunoperoxidase and immunogold labeling techniques, the localization of LICR-LON-E36 has been compared with that obtained with antibodies against the beta subunit of rat luteinizing hormone (beta LH) and human follicle stimulating hormone (beta FSH), porcine adrenocroticotropic hormone (ACTH) and rat S-100. LICR-LON-E36 is localized in a portion of beta LH-containing cells in female rat pituitaries and where present both antigens are localized within the same storage granules. LICR-LON-E36 is rarely detectable within beta LH-containing cells of male rat pituitaries that also stain positively with anti-beta FSH antibodies. ACTH and S-100 were localized within different cell populations.
利用光镜和电镜免疫细胞化学方法，对雄性和雌性大鼠树脂包埋垂体中由单克隆抗体LICR-LON-E36定位的抗原分布进行了研究。采用免疫过氧化物酶和免疫金标记技术，将LICR-LON-E36的定位与用抗大鼠促黄体生成素β亚基（β-LH）、人促卵泡生成素（β-FSH）、猪促肾上腺皮质激素（ACTH）和大鼠S-100抗体获得的定位进行了比较。LICR-LON-E36定位于雌性大鼠垂体中一部分含β-LH的细胞内，并且在存在的情况下，两种抗原都定位于同一储存颗粒内。在雄性大鼠垂体中含β-LH的细胞内很少能检测到LICR-LON-E36，而这些细胞用抗β-FSH抗体染色也呈阳性。ACTH和S-100定位于不同的细胞群体中。
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