Structure of a chromosomal Phaseolus vulgaris lectin gene and its transcript.

A genomic library of Phaseolus vulgaris DNA was screened using a lectin cDNA clone that includes the entire coding region to obtain a lambda clone containing the corresponding nuclear lectin gene. Comparison of the cDNA and genomic nucleotide sequences showed that the gene has no intervening sequences. The 5' untranslated region was found to be only 10-16 base pairs long by S1 nuclease mapping. The lectin transcriptional unit is flanked by sequences which are exceptionally high (72-75%) in A + T content and its 5' flanking region contains potential transcription control elements.