Department of Biotechnology, Institut Pasteur, 28 Rue du Docteur Roux, F-75724, Paris Cedex 15, France.
Plant Mol Biol. 1987 Sep;9(5):497-507. doi: 10.1007/BF00015881.
Molecular hybridization experiments have shown that the pea genome contains four regions which hybridize with pea lectin cDNA (Kaminski, Buffard, and Strosberg, 1986. Plant Science 46, 111-116). The complete organization of the pea lectin gene family was investigated. Four partial EcoRI genomic libraries were screened with a lectin cDNA (pPS 15-50) covering the entire coding region. Four positive recombinant phages, λI 101, λI 52, λIII 51 and λIV 22, were isolated and the DNA sequences of the subclones, designated respectively PSL1, PSL2, PSL3 and PSL4, were determined. PSL2, PSL3 and PSL4 are incomplete genes; the presence of several stop codons in the correct reading frames indicate that these genes cannot code for a functional lectin protein. The sequences of PSL1 and pPS 15-50 have identical coding regions. The pea lectin gene has no intervening sequences and is flanked at its 5' region by a sequence containing an exceptionally high A+T content (73%). Eucaryotic consensus sequences such as a TATA box and a polyadenylation signal are also found in the flanking regions of the PSL1 clone.
分子杂交实验表明,豌豆基因组含有四个区域与豌豆凝集素 cDNA 杂交(Kaminski、Buffard 和 Strosberg,1986. Plant Science 46, 111-116)。研究了豌豆凝集素基因家族的完整组织。用覆盖整个编码区的凝集素 cDNA (pPS 15-50)筛选了四个部分的 EcoRI 基因组文库。分离出四个阳性重组噬菌体 λI 101、λI 52、λIII 51 和 λIV 22,并确定了亚克隆的 DNA 序列,分别命名为 PSL1、PSL2、PSL3 和 PSL4。PSL2、PSL3 和 PSL4 是不完全基因;在正确的阅读框架中存在几个终止密码子表明这些基因不能编码功能性凝集素蛋白。PSL1 和 pPS 15-50 的序列具有相同的编码区。豌豆凝集素基因没有内含子,其 5' 区域侧翼序列含有异常高的 A+T 含量(73%)。在 PSL1 克隆的侧翼区域还发现了真核生物的保守序列,如 TATA 盒和多聚腺苷酸化信号。