Sugio K, Daly J W
Life Sci. 1984 Oct 8;35(15):1575-83. doi: 10.1016/0024-3205(84)90356-4.
Adenosine and various analogs potentiated plasma exudation elicited by bradykinin in rat skin using 125I-labelled bovine serum albumin (125I-BSA) as a tracer. L-N6-Phenylisopropyladenosine (L-PIA) was much more effective than D-PIA, adenosine, N6-cyclohexyladenosine (CHA) and 2-chloroadenosine, all of which were comparable in activity. Adenosine 5'-cyclopropylcarboxamide was the least effective analog. Caffeine and theophylline had no effect on basal or bradykinin-elicited plasma exudation, while inhibiting plasma exudation elicited by L-PIA, CHA or a combination of bradykinin and L-PIA. 8-Phenyltheophylline was more potent than caffeine or theophylline versus the bradykinin and L-PIA combination. 2',5'-Dideoxyadenosine, a P-site inhibitor of adenylate cyclase, had no effect on plasma exudation elicited by bradykinin, L-PIA or a combination of bradykinin and L-PIA, but did inhibit plasma exudation elicited by prostaglandin E1 (PGE1) or a bradykinin-PGE1-combination. The antihistamine cyproheptadine slightly reduced plasma exudation elicited by a bradykinin-PGE1 combination. The results suggest that adenosine potentiates bradykinin-induced plasma exudation via an adenosine receptor and that histamine may be involved to some extent in the phenomenon.
使用125I标记的牛血清白蛋白（125I-BSA）作为示踪剂，腺苷及多种类似物可增强缓激肽在大鼠皮肤中引发的血浆渗出。L-N6-苯基异丙基腺苷（L-PIA）比D-PIA、腺苷、N6-环己基腺苷（CHA）和2-氯腺苷的效果要强得多，后几种物质的活性相当。腺苷5'-环丙基甲酰胺是效果最差的类似物。咖啡因和茶碱对基础或缓激肽引发的血浆渗出没有影响，但可抑制L-PIA、CHA或缓激肽与L-PIA联合引发的血浆渗出。对于缓激肽和L-PIA联合作用，8-苯基茶碱比咖啡因或茶碱的作用更强。2'，5'-二脱氧腺苷是腺苷酸环化酶的P位点抑制剂，对缓激肽、L-PIA或缓激肽与L-PIA联合引发的血浆渗出没有影响，但可抑制前列腺素E1（PGE1）或缓激肽与PGE1联合引发的血浆渗出。抗组胺药赛庚啶可略微降低缓激肽与PGE1联合引发的血浆渗出。结果表明，腺苷通过腺苷受体增强缓激肽诱导的血浆渗出，并且组胺可能在一定程度上参与了该现象。
