Berger G, Verbaere J, Feroldi J
Ultrastruct Pathol. 1984;6(4):319-29. doi: 10.3109/01913128409018590.
Ultrastructural and immunohistochemical studies in a case of placental site trophoblastic tumor (PSTT) of the uterus were carried out in order to define the nature of the abnormal tissue. By electron microscopy, the large cells, whether mononuclear or syncytial, showed numerous ribosomes, prominent Golgi elements, and abundant rough endoplasmic reticulum (RER) filled with granular material. Pseudopods and microvilli were found on the cell surfaces. By immunofluorescence, the well-developed filamentous cytoskeleton proved to be actin-rich. beta-HCG (human chorionic gonadotropin) and SP1 (beta 1-specific pregnancy glycoprotein) were detected in only a few tumor cells, whereas most of them stained for HPL (human placental lactogen). The present results show the secretory nature of most of the tumor cells, which resemble the intermediate trophoblast of the placental bed. Together with previous studies, they suggest that a varying spectrum of syncytiotrophoblastic differentiation exists in PSTT. Decidual, myometrial, or histiocytic cells do not seem involved in the histogenesis of the tumor tissue.
对一例子宫胎盘部位滋养细胞肿瘤（PSTT）进行了超微结构和免疫组织化学研究，以明确异常组织的性质。通过电子显微镜观察，大细胞，无论是单核还是合体细胞，都显示出大量核糖体、显著的高尔基体成分以及充满颗粒物质的丰富粗面内质网（RER）。在细胞表面发现了伪足和微绒毛。通过免疫荧光检测，发育良好的丝状细胞骨架富含肌动蛋白。仅在少数肿瘤细胞中检测到β-HCG（人绒毛膜促性腺激素）和SP1（β1特异性妊娠糖蛋白），而大多数肿瘤细胞对HPL（人胎盘催乳素）呈阳性染色。目前的结果显示大多数肿瘤细胞具有分泌性质，类似于胎盘床的中间滋养细胞。与先前的研究一起，提示PSTT中存在不同程度的合体滋养细胞分化。蜕膜、肌层或组织细胞似乎不参与肿瘤组织的组织发生。
