Eshet R, Peleg S, Laron Z
Acta Endocrinol (Copenh). 1984 Sep;107(1):9-15. doi: 10.1530/acta.0.1070009.
The distribution of human growth hormone (hGH) receptor complexes on IM-9 cultured lymphocytes was studied using fluorescein and 125I-labelled hGH (F-hGH and [125I]hGH). The cells labelled with F-hGH were visualized with a sensitive video intensification microscopic system which permitted direct observation of the location of the fluorescent hormone on the surface of the living lymphocytes. At 4 degrees C F-hGH bound diffusely to the cell surface and remained dispersed but following incubation for 30 min at 37 degrees C the hormone receptor complexes aggregated into patches on the cell surface and formed a single cap on one pole of the cell. Progressive internalization into the cell was demonstrated at 37 degrees C with [125I]hGH. It is hypothesized that the aggregation and internalization of the hGH receptor complexes are associated with the action and degradation of the hormone and probably also with the mechanism of down-regulation of the receptors.
利用荧光素和125I标记的人生长激素（F-hGH和[125I]hGH）研究了IM-9培养淋巴细胞上人生长激素（hGH）受体复合物的分布。用灵敏的视频增强显微系统观察F-hGH标记的细胞，该系统可直接观察荧光激素在活淋巴细胞表面的位置。在4℃时，F-hGH扩散性地结合到细胞表面并保持分散状态，但在37℃孵育30分钟后，激素受体复合物在细胞表面聚集成斑块，并在细胞的一极形成单个帽状物。用[125I]hGH在37℃证明了其逐渐内化进入细胞。据推测，hGH受体复合物的聚集和内化与激素的作用和降解有关，可能也与受体下调机制有关。
