Shak S, Goldstein I M
Biochem Biophys Res Commun. 1984 Sep 17;123(2):475-81. doi: 10.1016/0006-291x(84)90255-9.
Carbon monoxide significantly inhibits omega-oxidation of exogenous leukotriene B4 to 20-OH-leukotriene B4 and 20-COOH-leukotriene B4 by unstimulated polymorphonuclear leukocytes as well as omega-oxidation of leukotriene B4 that is generated when cells are stimulated with the calcium ionophore, A23187. Inhibition of omega-oxidation by carbon monoxide is concentration-dependent, completely reversible, and specific. Carbon monoxide does not affect synthesis of leukotriene B4 by stimulated polymorphonuclear leukocytes or other cell functions (i.e., degranulation, superoxide anion generation). These findings suggest that a cytochrome P-450 enzyme in human polymorphonuclear leukocytes is responsible for catabolizing leukotriene B4 by omega-oxidation.
一氧化碳可显著抑制外源性白三烯B4被未受刺激的多形核白细胞ω-氧化为20-羟基白三烯B4和20-羧基白三烯B4，以及细胞被钙离子载体A23187刺激时所产生的白三烯B4的ω-氧化。一氧化碳对ω-氧化的抑制作用具有浓度依赖性、完全可逆且具有特异性。一氧化碳不影响受刺激的多形核白细胞对白三烯B4的合成或其他细胞功能（即脱颗粒、超氧阴离子生成）。这些发现表明，人类多形核白细胞中的一种细胞色素P-450酶负责通过ω-氧化来分解代谢白三烯B4。
