Chambers T J, Fuller K, Athanasou N A
Br J Exp Pathol. 1984 Oct;65(5):557-66.
We isolated osteoclasts from neonatal rat bone and assessed the effects of prostaglandins (PGs) I2, E1 and E2 on osteoclastic spreading. We found that although the PGs and dibutyryl cyclic AMP (DB-cAMP) acted as direct inhibitors of osteoclastic spreading, if osteoblasts and osteoclasts were co-cultured, the addition of PGs or DB-cAMP caused a considerable increase in spreading. This suggests that the PGs and DB-cAMP induce osteoblasts to stimulate osteoclasts. Osteoblasts are known to produce PGs, and thus possess the capacity to either inhibit (through PGs) or stimulate osteoclasts. Our results suggest that the balance between stimulation and inhibition of osteoclasts by osteoblasts may be determined by osteoblastic cyclic AMP levels.
我们从新生大鼠骨骼中分离出破骨细胞，并评估了前列腺素（PGs）I2、E1和E2对破骨细胞铺展的影响。我们发现，尽管PGs和二丁酰环磷腺苷（DB-cAMP）可作为破骨细胞铺展的直接抑制剂，但如果将成骨细胞和破骨细胞共同培养，添加PGs或DB-cAMP会导致铺展显著增加。这表明PGs和DB-cAMP诱导成骨细胞刺激破骨细胞。已知成骨细胞会产生PGs，因此具有抑制（通过PGs）或刺激破骨细胞的能力。我们的结果表明，成骨细胞对破骨细胞刺激与抑制之间的平衡可能由成骨细胞的环磷腺苷水平决定。
