McLoughlin P M, Sun I L, Crane F L
Biochim Biophys Acta. 1984 Nov 6;802(1):71-6. doi: 10.1016/0304-4165(84)90035-7.
Transplasma membrane electron transport, as assayed by external ferricyanide reduction, has been related to control of growth and hormone response of cells. Elicitor-stimulated transmembrane NADPH oxidase is important for bacteriocidal superoxide production by neutrophils. Since adriamycin is myelosuppressive and can stimulate superoxide production, its effects on the two redox systems of porcine neutrophil plasma membranes were compared. Adriamycin inhibits transplasma membrane ferricyanide and stimulates superoxide production activated by phorbol myristate acetate (PMA). Ferricyanide reduction in PMA-treated cells becomes resistant to inhibition by adriamycin. These results provide evidence for an independent effect of adriamycin on transmembrane ferricyanide reduction and on superoxide generation.
通过外部铁氰化物还原测定的跨质膜电子传递与细胞生长控制及激素反应有关。激发子刺激的跨膜NADPH氧化酶对于中性粒细胞产生杀菌性超氧化物很重要。由于阿霉素具有骨髓抑制作用且能刺激超氧化物产生，因此比较了其对猪中性粒细胞质膜两个氧化还原系统的影响。阿霉素抑制跨质膜铁氰化物还原，并刺激佛波酯（PMA）激活的超氧化物产生。PMA处理的细胞中的铁氰化物还原对阿霉素的抑制产生抗性。这些结果为阿霉素对跨膜铁氰化物还原和超氧化物生成的独立作用提供了证据。
