Rainbow T C, Wieczorek C M, Halpain S
Brain Res. 1984 Aug 20;309(1):173-7. doi: 10.1016/0006-8993(84)91025-4.
Binding sites for the potent glutamate agonist [3H] alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) were localized in rat brain frozen sections by quantitative autoradiography. Highest levels of binding were seen in stratum radiatum and stratum oriens of the CA1 hippocampal subfield and in the dorsal subiculum. Substantially less but still high amounts of [3H]AMPA binding occurred in other hippocampal subfields and in rostral forebrain structures. The heterogeneous nature of [3H]AMPA binding is discussed in relation to [3H]glutamate binding visualized by similar methods. From these data it is suggested that [3H]AMPA may label a particular subclass of the glutamate receptor population which exhibits a high affinity for quisqualic acid.
通过定量放射自显影法，将强效谷氨酸激动剂[3H]α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）的结合位点定位在大鼠脑冰冻切片中。在海马CA1亚区的辐射层和海马下脚以及背侧海马旁回中观察到最高水平的结合。在其他海马亚区和前脑嘴侧结构中，[3H]AMPA结合量明显较少，但仍处于较高水平。结合通过类似方法观察到的[3H]谷氨酸结合情况，讨论了[3H]AMPA结合的异质性。从这些数据推测，[3H]AMPA可能标记了对quisqualic酸具有高亲和力的谷氨酸受体群体中的一个特定亚类。
