Ledger R, Stellwagen E
J Chromatogr. 1984 Sep 7;299(1):175-83. doi: 10.1016/s0021-9673(01)97830-6.
A variety of aminoalkylated microparticulate porous silicas have been prepared and used to displace the reactive chloride from reactive blue 2 thereby immobilizing the dye in a mild and predictable manner. Methods of analysis of immobilized reactive blue 2 are compared and a rapid new method is recommended which is clean and non-destructive. The biospecific elution of a dehydrogenase from an immobilized dye column is demonstrated.
已制备了多种氨烷基化的微粒状多孔硅胶，并用于从活性蓝2中置换出反应性氯，从而以温和且可预测的方式固定染料。比较了固定化活性蓝2的分析方法，并推荐了一种快速、清洁且无损的新方法。展示了从固定化染料柱上对脱氢酶进行生物特异性洗脱的过程。
