Averett D R, Furman P A, Spector T
J Virol. 1984 Dec;52(3):981-3. doi: 10.1128/JVI.52.3.981-983.1984.
The ribonucleotide reductase (ribonucleoside-diphosphate reductase; EC 1.17.4.1) induced by herpes simplex virus type 2 infection of serum-starved BHK-21 cells was purified to provide a preparation practically free of both eucaryotic ribonucleotide reductase and contaminating enzymes that could significantly deplete the substrates. Certain key properties of the herpes simplex virus type 2 ribonucleotide reductase were examined to define the extent to which it resembled the herpes simplex virus type 1 ribonucleotide reductase. The herpes simplex virus type 2 ribonucleotide reductase was inhibited by ATP and MgCl2 but only weakly inhibited by the ATP X Mg complex. Deoxynucleoside triphosphates were at best only weak inhibitors of this enzyme. ADP was a competitive inhibitor (K'i, 11 microM) of CDP reduction (K'm, 0.5 microM), and CDP was a competitive inhibitor (K'i, 0.4 microM) of ADP reduction (K'm, 8 microM). These key properties closely resemble those observed for similarly purified herpes simplex virus type 1 ribonucleotide reductase and serve to distinguish these virally induced enzymes from other ribonucleotide reductases.
通过用2型单纯疱疹病毒感染血清饥饿的BHK - 21细胞诱导产生的核糖核苷酸还原酶（核糖核苷二磷酸还原酶；EC 1.17.4.1）被纯化，以获得一种实际上不含真核核糖核苷酸还原酶和可能显著消耗底物的污染酶的制剂。研究了2型单纯疱疹病毒核糖核苷酸还原酶的某些关键特性，以确定其与1型单纯疱疹病毒核糖核苷酸还原酶的相似程度。2型单纯疱疹病毒核糖核苷酸还原酶受到ATP和MgCl2的抑制，但仅受到ATP·Mg复合物的微弱抑制。脱氧核苷三磷酸充其量只是该酶的弱抑制剂。ADP是CDP还原（K'm，0.5 microM）的竞争性抑制剂（K'i，11 microM），而CDP是ADP还原（K'm，8 microM）的竞争性抑制剂（K'i，0.4 microM）。这些关键特性与同样纯化的1型单纯疱疹病毒核糖核苷酸还原酶所观察到的特性非常相似，有助于将这些病毒诱导的酶与其他核糖核苷酸还原酶区分开来。
