Fung L W, Ostrowski M S
Life Sci. 1984 Nov 12;35(20):2071-8. doi: 10.1016/0024-3205(84)90565-4.
Our earlier spin label electron paramagnetic resonance (EPR) studies of hereditary spherocytosis (HS) erythrocyte revealed the existence of structural alteration(s) when the membrane is subjected to heat stress. We have now used saturation transfer EPR to show restricted motion in membrane proteins even without subjecting membrane to stress. The abnormal motional behavior is amplified when membranes are incubated at 47 degrees C and is readily detectable by conventional EPR. Gel electrophoresis and lipid assays show that proteins but not lipids are released upon heating. Thus, the more restricted motions in HS membranes may be due to a different membrane protein organization, ultimately resulting in the abnormal morphology of HS cells.
我们早期对遗传性球形红细胞增多症（HS）红细胞进行的自旋标记电子顺磁共振（EPR）研究表明，当细胞膜受到热应激时会存在结构改变。我们现在使用饱和转移EPR来表明，即使在不对细胞膜施加应激的情况下，膜蛋白的运动也受到限制。当细胞膜在47摄氏度下孵育时，这种异常的运动行为会被放大，并且通过传统的EPR很容易检测到。凝胶电泳和脂质分析表明，加热时释放的是蛋白质而不是脂质。因此，HS细胞膜中运动受限可能是由于不同的膜蛋白组织，最终导致HS细胞出现异常形态。
