Pech I V, Stahl W L
Neurochem Res. 1984 Jun;9(6):757-69. doi: 10.1007/BF00965664.
Conditions have been established which allow growth of embryonic rat retinal cells in dissociated cell culture for up to one month. Na+, K+-ATPase localization was studied in both neuronal and mixed neuronal-glial (flat cell) cultures. High Na+, K+-ATPase-like-immunoreactivity was associated with plasma membranes of neuronal cell bodies and their processes. Markedly lower immunoreactivity was found in the underlying flat cells in mixed cultures. Staining was generally uniform over perikaryal plasma membranes and showed a bead-like appearance in neuronal processes, supporting previous studies in brain tissue which used histocytochemical procedures specific for the Na+, K+-ATPase. This system should be useful for examining distribution of the enzyme in developing nerve and glial cells and may help to resolve questions regarding Na+-K+ homeostasis by neurons and glia.
已经建立了一些条件，使得胚胎大鼠视网膜细胞在解离细胞培养中能够生长长达一个月。在神经元培养物和混合神经元 - 神经胶质（扁平细胞）培养物中研究了Na +，K + -ATP酶的定位。高Na +，K + -ATP酶样免疫反应性与神经元细胞体及其突起的质膜相关。在混合培养物中下层的扁平细胞中发现免疫反应性明显较低。染色在核周质膜上通常是均匀的，并且在神经元突起中呈现珠状外观，这支持了先前在脑组织中使用对Na +，K + -ATP酶特异的组织细胞化学程序的研究。该系统对于检查该酶在发育中的神经和神经胶质细胞中的分布应该是有用的，并且可能有助于解决关于神经元和神经胶质细胞的Na + -K +稳态的问题。
