Barg J, Levy R, Simantov R
Neurosci Lett. 1984 Sep 7;50(1-3):133-7. doi: 10.1016/0304-3940(84)90475-0.
Neuroblastoma-glioma NG108-15 cells that were cultured for 48 h with the opiate antagonist, naloxone, respond to the guanosine 5'-triphosphate (GTP) analogue guanosine 5'-[beta, gamma-imido]-triphosphate (GMP-PNP) in the binding assay as the control, non-treated, cells. This was observed when the guanyl nucleotide was tested in the presence or absence of sodium chloride and also after subcellular fractionation of the membranes on a sucrose gradient which separated between two receptor-containing fractions. The findings suggest that the increase in delta type enkephalin receptors in naloxone-treated NG108-15 cells does not reflect an alteration in the interaction between the receptor and the adenylate cyclase-GTP-binding protein system.
用阿片拮抗剂纳洛酮培养48小时的神经母细胞瘤-胶质瘤NG108-15细胞，在结合试验中对鸟苷5'-三磷酸（GTP）类似物鸟苷5'-[β,γ-亚氨基]-三磷酸（GMP-PNP）的反应与对照未处理细胞相同。当在有或没有氯化钠存在的情况下测试鸟苷酸，以及在蔗糖梯度上对膜进行亚细胞分级分离（分离出两个含受体的部分）后，均观察到这一现象。这些发现表明，纳洛酮处理的NG108-15细胞中δ型脑啡肽受体的增加并不反映受体与腺苷酸环化酶-GTP结合蛋白系统之间相互作用的改变。
