John M, Schmidt J
Anal Biochem. 1984 Sep;141(2):466-71. doi: 10.1016/0003-2697(84)90072-1.
Hydroxyapatite chromatography has been used to separate all five isozymes of lactic dehydrogenase, six enzymatically active forms of bovine pancreatic DNase I, and a standard protein mixture. The proteins were eluted with a linear gradient of sodium phosphate. Enzyme activity recoveries were greater than 90%. Packing materials were obtained from commercial fine-particle-sized hydroxyapatite (DNA grade Bio-Gel HTP) by an elutriation procedure. Long columns packed with small crystals were run under low pressure at acceptable flow rates, and were used over prolonged periods.
羟基磷灰石色谱法已被用于分离乳酸脱氢酶的所有五种同工酶、牛胰腺DNA酶I的六种酶活性形式以及一种标准蛋白质混合物。蛋白质用磷酸钠线性梯度洗脱。酶活性回收率大于90%。填充材料通过淘析程序从商业细粒度羟基磷灰石（DNA级Bio-Gel HTP）获得。填充有小晶体的长柱在低压下以可接受的流速运行，并可长期使用。
