Demotes-Mainard F, Vinçon G, Bouchet J L, Jarry C, Albin H
Ann Biol Clin (Paris). 1984;42(4):301-5.
The authors propose an assay technique for cefotaxime and desacetylcefotaxime in the plasma and urine, using high performance liquid chromatography. The proteins are precipitated by adding propanol-2 to 100 microliter of plasma or urine. After centrifugation, the supernatant is extracted by a mixture of chloroform and isoamyl alcohol. The cephalosporins remain in the superior aqueous phase and a fraction of this phase is injected into the chromatograph. The cefotaxime, desacetylcefotaxime and cephaloridine (internal standard) are separated on a Radial Pak C 18 column by ion-binding chromatography. The mobile phase consists of a mixture of distilled water and acetonitrile (830/170, V/V) with the addition of an ampoule of Pic A (tetrabutylammonium phosphate). This rapid, specific and sensitive micro-method can be used for the assay of these antibiotics in adults and children, for therapeutic monitoring and for pharmacokinetic studies.
作者提出了一种利用高效液相色谱法测定血浆和尿液中头孢噻肟和去乙酰头孢噻肟的检测技术。向100微升血浆或尿液中加入异丙醇使蛋白质沉淀。离心后，上清液用氯仿和异戊醇的混合物萃取。头孢菌素保留在上层水相中，取该相的一部分注入色谱仪。头孢噻肟、去乙酰头孢噻肟和头孢啶（内标）通过离子结合色谱法在Radial Pak C 18柱上分离。流动相由蒸馏水和乙腈（830/170，V/V）的混合物组成，并添加一支Pic A（磷酸四丁铵）。这种快速、特异且灵敏的微量方法可用于成人和儿童这些抗生素的测定、治疗监测及药代动力学研究。
