毒蕈碱受体刺激增强豚鼠回肠平滑肌中的多磷酸肌醇分解。
Muscarinic-receptor stimulation enhances polyphosphoinositide breakdown in guinea-pig ileum smooth muscle.
作者信息
Sekar M C, Roufogalis B D
出版信息
Biochem J. 1984 Oct 15;223(2):527-31. doi: 10.1042/bj2230527.
Abstract
Muscarinic-receptor stimulation by 0.1 mM-carbachol in longitudinal muscle of the guinea-pig ileum increases the incorporation of [3H]inositol into inositol-containing phospholipid. This effect was blocked by 16 microM-atropine. After 60 min incubation, carbachol increased the accumulation of total inositol phosphates 20-fold in the presence of 10 mM-Li+. Less than 20% of the total inositol phosphate corresponded to inositol 1-phosphate by ion-exchange chromatography, whereas of the remainder about two-thirds corresponded to inositol bisphosphate and one third to inositol trisphosphate. It is concluded that stimulation of muscarinic receptors in guinea-pig ileum enhances breakdown of polyphosphoinositides, suggesting that this may be a primary event associated with Ca2+ mobilization in the guinea-pig ileum.
摘要
在豚鼠回肠纵行肌中，0.1 mM 卡巴胆碱对毒蕈碱受体的刺激可增加[3H]肌醇掺入含肌醇磷脂的量。这种效应被16 μM 阿托品阻断。孵育60分钟后，在10 mM 锂离子存在的情况下，卡巴胆碱使总肌醇磷酸的积累增加了20倍。通过离子交换色谱法，总肌醇磷酸中不到20%对应于肌醇1-磷酸，而其余部分中约三分之二对应于肌醇二磷酸，三分之一对应于肌醇三磷酸。得出的结论是，豚鼠回肠中毒蕈碱受体的刺激增强了多磷酸肌醇的分解，这表明这可能是与豚鼠回肠中钙离子动员相关的一个主要事件。
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