Shaw C, Needler M C, Cynader M
Brain Res Bull. 1984 Aug;13(2):331-4. doi: 10.1016/0361-9230(84)90134-5.
In vitro receptor binding techniques were used to study the characteristics, distribution, and ontogenesis of muscimol binding sites in cat visual cortex. [3H] Muscimol, a GABA agonist, labelled a single population of binding sites with a KD of 18 nM in adult cats. Specific binding was saturable, reversible and was blocked by the addition of GABA or (+) bicuculline. Autoradiograms revealed that the highest density of [3H] muscimol binding occurred in cortical layer IV. Similar patterns of [3H] muscimol binding were observed at all ages examined, although the binding densities differed. The peak [3H] muscimol binding density, corrected for amount of protein, occurred at 3 months postnatally. In 3 day old and adult cats binding density was 41% and 69%, respectively, of the peak value.
采用体外受体结合技术研究猫视觉皮层中蝇蕈醇结合位点的特征、分布及个体发生。[3H]蝇蕈醇是一种γ-氨基丁酸（GABA）激动剂，在成年猫中标记了单一的结合位点群体，其解离常数（KD）为18 nM。特异性结合具有饱和性、可逆性，并且可通过添加GABA或（+）荷包牡丹碱来阻断。放射自显影显示，[3H]蝇蕈醇结合的最高密度出现在皮层第IV层。在所检查的所有年龄段均观察到类似的[3H]蝇蕈醇结合模式，尽管结合密度有所不同。经蛋白质含量校正后的[3H]蝇蕈醇结合密度峰值出现在出生后3个月。在3日龄和成年猫中，结合密度分别为峰值的41%和69%。
