Kelln R A
Can J Microbiol. 1984 Aug;30(8):991-6. doi: 10.1139/m84-154.
Transconjugants were not recovered in matings between Salmonella typhimurium and Escherichia coli K-12 strains carrying the chromosomal region between leu and argF on an F-prime factor, even when a restriction-deficient recipient was used. A mutant F-prime factor compatible for transfer to S. typhimurium was constructed by transposon mutagenesis and characterized as being deficient in directing the synthesis of UMP kinase (encoded by pyrH). Other compatible F-prime factors were readily constructed by employing a procedure designed to select for strains carrying F-prime factors harboring pyrH mutations.
即使使用了限制缺陷型受体，在鼠伤寒沙门氏菌与携带F-prime因子上亮氨酸和精氨酸F之间染色体区域的大肠杆菌K-12菌株进行交配时，也未获得转接合子。通过转座子诱变构建了一种可与鼠伤寒沙门氏菌转移兼容的突变F-prime因子，其特征是在指导UMP激酶（由pyrH编码）的合成方面存在缺陷。通过采用一种旨在选择携带具有pyrH突变的F-prime因子的菌株的程序，很容易构建出其他兼容的F-prime因子。
