Parvinen M, Nikula H, Huhtaniemi I
Mol Cell Endocrinol. 1984 Oct;37(3):331-6. doi: 10.1016/0303-7207(84)90103-5.
The interaction of seminiferous tubules and Leydig cells was investigated in the rat in vitro. Crude collagenase-dispersed, or Percoll-purified, Leydig cells were incubated together with seminiferous tubules at different stages of the cycle, isolated by transillumination-assisted microdissection. The testosterone production was measured. Seminiferous tubules inhibited testosterone production of crude Leydig cell preparations, but induced a clear stimulation (30-100%) in Percoll-purified cells. The stimulation was maximal at stages VII and VIII of the cycle, significantly higher than at stages II-VI (P less than 0.05). The stimulation by seminiferous tubules was observed both in basal and hCG-stimulated testosterone production. The effect was independent of FSH or GnRH action. These results demonstrate the presence of paracrine regulatory interaction between seminiferous tubules and Leydig cells, and are in agreement with the concept of a preferential androgen requirement of stages VII and VIII of the cycle.
在体外对大鼠的生精小管和睾丸间质细胞之间的相互作用进行了研究。用粗制胶原酶分散或经Percoll纯化的睾丸间质细胞与通过透照辅助显微切割分离的处于不同周期阶段的生精小管一起孵育。检测睾酮的产生。生精小管抑制粗制睾丸间质细胞制剂的睾酮产生，但对经Percoll纯化的细胞有明显的刺激作用（30% - 100%）。这种刺激在周期的VII和VIII阶段最大，显著高于II - VI阶段（P < 0.05）。在基础睾酮产生和人绒毛膜促性腺激素（hCG）刺激的睾酮产生中均观察到生精小管的刺激作用。该作用独立于促卵泡激素（FSH）或促性腺激素释放激素（GnRH）的作用。这些结果证明了生精小管和睾丸间质细胞之间存在旁分泌调节相互作用，并且与周期VII和VIII阶段对雄激素的优先需求概念一致。
