来自多变鱼腥藻的限制性内切核酸酶对克隆的多变鱼腥藻DNA的切割频率较低。
Infrequent cleavage of cloned Anabaena variabilis DNA by restriction endonucleases from A. variabilis.
作者信息
Herrero A, Elhai J, Hohn B, Wolk C P
出版信息
J Bacteriol. 1984 Nov;160(2):781-4. doi: 10.1128/jb.160.2.781-784.1984.
Abstract
A cosmid vector has been constructed, using a lambda replicon. A library of cosmids from Anabaena variabilis ATCC 29413 based on use of this vector is shown to be highly deficient in sites for the two type II restriction endonucleases found in that organism.
摘要
已构建了一种使用λ复制子的黏粒载体。基于该载体构建的多变鱼腥藻ATCC 29413的黏粒文库显示，该文库中该生物体所含的两种II型限制性内切核酸酶的切割位点严重缺失。
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