人重组干扰素和淋巴母细胞样干扰素在人源及异源细胞系中的活性

Activity of human recombinant and lymphoblastoid interferons in human and heterologous cell lines.

作者信息

Overall J C, Yeh T J, Kern E R

出版信息

J Interferon Res. 1984 Fall;4(4):529-33. doi: 10.1089/jir.1984.4.529.

DOI:10.1089/jir.1984.4.529

Abstract

Three human alpha interferon (HuIFN-alpha) preparations currently being used in clinical trials, rIFN-alpha A, rIFN-alpha 2, and lymphoblastoid IFN (LYM-IFN) had appreciable activity against encephalomyocarditis and vesicular stomatitis viruses (VSV) in guinea pig transformed and guinea pig embryo cells, but not mouse L or rabbit kidney cells. The level of activity in guinea pig cells, compared with human WISH cells, was 182% (range 9%-1,900%). These results suggest that the guinea pig may be useful for testing the antiviral, anticancer, or immunomodulatory activity of Hu alpha IFNs in vivo.

摘要

目前正在临床试验中使用的三种人α干扰素（HuIFN-α）制剂，即重组干扰素αA、重组干扰素α2和淋巴母细胞干扰素（LYM-IFN），在豚鼠转化细胞和豚鼠胚胎细胞中对脑心肌炎病毒和水疱性口炎病毒（VSV）具有明显活性，但在小鼠L细胞或兔肾细胞中则无活性。与人类WISH细胞相比，在豚鼠细胞中的活性水平为182%（范围为9%-1900%）。这些结果表明，豚鼠可能有助于在体内测试HuαIFN的抗病毒、抗癌或免疫调节活性。