通过电子显微镜观察环磷酸腺苷受体蛋白诱导的DNA扭结
Visualization of cAMP receptor protein-induced DNA kinking by electron microscopy.
作者信息
Gronenborn A M, Nermut M V, Eason P, Clore G M
出版信息
J Mol Biol. 1984 Nov 15;179(4):751-7. doi: 10.1016/0022-2836(84)90166-9.
The effect of specific DNA binding of the cAMP . cAMP receptor protein complex to two DNA fragments (301 and 2685 base-pairs in length) containing the lac operon has been investigated by electron microscopy. It is shown that specific DNA binding of the cAMP . cAMP receptor protein complex induces a kink of 30 to 45 degrees in the DNA with the apex of the kink located at the site of protein attachment. These findings lend direct visual support for the kinking hypothesis based on the observation of anomalous electrophoretic mobility of DNA fragments containing specifically bound cAMP receptor protein.
通过电子显微镜研究了环磷酸腺苷（cAMP）. cAMP受体蛋白复合物与包含乳糖操纵子的两个DNA片段（长度分别为301和2685个碱基对）的特异性DNA结合作用。结果表明，cAMP. cAMP受体蛋白复合物的特异性DNA结合会使DNA产生30至45度的弯折，弯折的顶点位于蛋白质附着位点。这些发现为基于对含有特异性结合cAMP受体蛋白的DNA片段异常电泳迁移率的观察而提出的弯折假说提供了直接的视觉支持。
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