Inamori Y, Kato Y, Kubo M, Nakanishi J, Nakashima M, Gemba M
Jpn J Pharmacol. 1984 Aug;35(4):397-401. doi: 10.1254/jjp.35.397.
In vitro effects of racemomycin-D on cellular metabolism were examined in rat kidney. Racemomycin-D decreased the concentration gradient of Na+ and K+ across the cell membranes, but failed to influence water content and ATP concentration of kidney cortical slices. The antibiotic inhibited microsomal (Na+ + K+)-ATPase. Preincubation of the microsomes with racemomycin-D enhanced the inhibition about 1.8-fold. Succinoxidase activity of mitochondria remained unaltered in the presence of racemomycin-D, but the antibiotic potently decreased ATP-dependent Ca2+ and Mg2+ uptakes by mitochondria. These results suggest that racemomycin-D probably disorders intracellular homeostasis of Na+, K+ and Ca2+.
在大鼠肾脏中检测了消旋霉素-D对细胞代谢的体外作用。消旋霉素-D降低了细胞膜两侧Na⁺和K⁺的浓度梯度，但未能影响肾皮质切片的含水量和ATP浓度。该抗生素抑制微粒体（Na⁺ + K⁺）-ATP酶。微粒体与消旋霉素-D预孵育可使抑制作用增强约1.8倍。在消旋霉素-D存在的情况下，线粒体的琥珀酸氧化酶活性保持不变，但该抗生素显著降低了线粒体对ATP依赖性Ca²⁺和Mg²⁺的摄取。这些结果表明，消旋霉素-D可能扰乱细胞内Na⁺、K⁺和Ca²⁺的稳态。
