Gil A, Proudfoot N J
Nature. 1984;312(5993):473-4. doi: 10.1038/312473a0.
The sequence AAUAAA, found 11-30 base pairs (bp) upstream of the poly(A) site of most non-histone eukaryotic messenger RNAs (mRNAs) forms an essential part of the recognition site for 3'-end processing of the primary transcript. However, the sequence AATAAA is found in transcribed regions of genes and is differentially utilized in genes containing multiple copies of the sequence within the 3'-noncoding region, suggesting that the hexanucleotide alone does not comprise a complete recognition site. Therefore, it seems likely that additional sequences are required to form a complete recognition site for 3'-end formation. We have investigated the sequence requirements for mRNA 3'-end formation using the rabbit beta-globin gene as a model system. Here we demonstrate that an additional sequence 3' to AAUAAA is required for the correct 3'-end formation of rabbit beta-globin mRNA.
AAUAAA序列位于大多数非组蛋白真核生物信使核糖核酸（mRNA）的聚腺苷酸化位点上游11 - 30个碱基对（bp）处，它构成了初级转录本3'端加工识别位点的重要组成部分。然而，AATAAA序列存在于基因的转录区域，并且在3'非编码区域包含该序列多个拷贝的基因中被差异利用，这表明仅六核苷酸并不构成完整的识别位点。因此，似乎需要额外的序列来形成完整的3'端形成识别位点。我们以兔β-珠蛋白基因为模型系统研究了mRNA 3'端形成的序列要求。在此我们证明，兔β-珠蛋白mRNA正确的3'端形成需要AAUAAA下游的额外序列。
