McMahon M E, Stamenkovich D, Petes T D
Nucleic Acids Res. 1984 Nov 12;12(21):8001-16. doi: 10.1093/nar/12.21.8001.
Most of the ribosomal RNA genes of the yeast Saccharomyces cerevisiae are about 9 kilobases (kb) in size and encode both the 35S rRNA (processed to produce the 25S, 18S, and 5.8S species) and 5S rRNA. These genes are arranged in a single tandem array of 100 repeats. Below, we present evidence that at the centromere-distal end of this array is a tandem arrangement of a different type of rRNA gene. Each of these repeats is 3.6 kb in length and encodes a single 5S rRNA. The coding sequence of this gene is different from that of the "normal" 5S gene in three positions located at the 3' end of the gene.
酿酒酵母的大多数核糖体RNA基因大小约为9千碱基(kb),编码35S rRNA(加工后产生25S、18S和5.8S种类)和5S rRNA。这些基因排列成一个由100个重复序列组成的单一串联阵列。下面,我们提供证据表明,在该阵列的着丝粒远端是一种不同类型rRNA基因的串联排列。这些重复序列中的每一个长度为3.6 kb,编码单个5S rRNA。该基因的编码序列在位于基因3'端的三个位置与“正常”5S基因不同。