采用不同底物的方法测定正常血压和高血压受试者血浆中血管紧张素转换酶的色谱洗脱图谱。

Chromatographic elution profiles of angiotensin converting enzyme measured by the methods using different substrates in the plasma from normotensive and hypertensive subjects.

作者信息

Otokida K, Nakamura M, Kawamura K, Kanazawa S, Kato M

出版信息

Tohoku J Exp Med. 1984 Sep;144(1):91-101. doi: 10.1620/tjem.144.91.

DOI:10.1620/tjem.144.91

Abstract

The angiotensin converting enzyme (ACE) activity in the plasma collected from normotensive and hypertensive patients was measured chromatographically by two different methods using hippuryl-histidyl-leucine (HHL-ACE) and synthetic angiotensin I (AI-ACE) as substrates. A single peak was observed in the elution profiles of HHL-ACE on both Sephadex G 150 column and DEAE Sephadex A 50 cellulose column chromatographies. A single peak of AI-ACE appeared on the gel-filtration. However, several peaks of AI-ACE were seen on DEAE Sephadex A 50 cellulose column chromatography, suggesting that there are various types of ACE which hydrolyze angiotensin I but not HHL.

摘要

采用两种不同的色谱法，分别以马尿酰 - 组氨酰 - 亮氨酸（HHL - ACE）和合成血管紧张素I（AI - ACE）为底物，测定了从血压正常和高血压患者采集的血浆中血管紧张素转换酶（ACE）的活性。在Sephadex G 150柱和DEAE Sephadex A 50纤维素柱色谱上，HHL - ACE的洗脱图谱均观察到一个单一峰。在凝胶过滤中出现了AI - ACE的一个单一峰。然而，在DEAE Sephadex A 50纤维素柱色谱上可见多个AI - ACE峰，这表明存在多种能水解血管紧张素I但不能水解HHL的ACE类型。