Letch C A
Trans R Soc Trop Med Hyg. 1984;78(5):627-30. doi: 10.1016/0035-9203(84)90223-2.
Cloning trypanosomes clearly showed that at least two enzymically distinct populations of Trypanozoon were present within the salivary glands of a wild-caught Glossina palpalis palpalis from Ivory Coast. Normal sampling techniques detected only the predominant population, which would be the only population reaching a stage suitable for enzyme characterization. Thus a tsetse fly may fail to be implicated as a transmitter of man-infective trypanosomes. The enzymic relationship of the two Trypanozoon populations suggested genetic hybridization. The separate clones retained their individual enzymic integrity after blood and cyclic transmission.
克隆锥虫清楚地表明,在从象牙海岸野生捕获的罗氏采采蝇的唾液腺中,至少存在两种酶学性质不同的布氏锥虫群体。常规采样技术仅检测到占主导地位的群体,而这将是唯一达到适合进行酶特性鉴定阶段的群体。因此,采采蝇可能不会被认为是人类感染性锥虫的传播媒介。这两种布氏锥虫群体的酶学关系表明存在基因杂交。在血液传播和周期性传播后,分离出的克隆体保持了它们各自的酶学完整性。