来自嗜热细菌PS3的脉冲细胞色素c氧化酶。
Pulsed cytochrome c oxidase from the thermophilic bacterium PS3.
作者信息
Sone N, Naqui A, Kumar C, Chance B
出版信息
Biochem J. 1984 Nov 1;223(3):809-13. doi: 10.1042/bj2230809.
A caa3-type terminal cytochrome c oxidase (EC 1.9.3.1) from the thermophilic bacterium PS3 containing three subunits showed conversion from resting into pulsed form. Upon pulsing (reduction and re-oxidation), the cytochrome c oxidase activity increased over 10-fold. This enhanced activity of the pulsed enzyme gradually decayed. Addition of phospholipids, necessary for the enzyme activity, did not affect this decay process. Small changes in the absorption spectrum were observed for the resting-into-pulsed transition and for H2O2 ligation to the pulsed enzyme. The e.p.r. spectrum of the resting enzyme was very similar to that of mitochondrial enzyme, but the transient g = 5, 1.78 and 1.69 set of e.p.r. signals, associated with the pulsed bovine heart oxidase, were not observed in the case of pulsed bacterium-PS3 enzyme.
来自嗜热细菌PS3的一种含三个亚基的caa3型末端细胞色素c氧化酶（EC 1.9.3.1）呈现出从静止形式向脉冲形式的转变。在脉冲处理（还原和再氧化）后，细胞色素c氧化酶活性增加了10倍以上。这种脉冲酶增强的活性逐渐衰减。添加酶活性所必需的磷脂并不影响这种衰减过程。在从静止到脉冲的转变以及过氧化氢与脉冲酶结合时观察到吸收光谱有微小变化。静止酶的电子顺磁共振光谱与线粒体酶的非常相似，但在脉冲细菌PS3酶的情况下未观察到与脉冲牛心氧化酶相关的瞬态g = 5、1.78和1.69的电子顺磁共振信号组。
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