褐球固氮菌7Fe铁氧化还原蛋白。还原型3Fe簇的两种pH依赖性形式及其向4Fe簇的转化。
Azotobacter chroococcum 7Fe ferredoxin. Two pH-dependent forms of the reduced 3Fe clusters and its conversion to a 4Fe cluster.
作者信息
George S J, Richards A J, Thomson A J, Yates M G
出版信息
Biochem J. 1984 Nov 15;224(1):247-51. doi: 10.1042/bj2240247.
Abstract
Ferredoxin from Azotobacter chroococcum has been studied by low-temperature magnetic-circular-dichroism and electron-paramagnetic-resonance spectroscopy. When aerobically isolated ferredoxin contains a [3Fe-4S] and [4Fe-4S] cluster. Anaerobic treatment with dithionite in the presence of ethanediol reduces the [3Fe-4S] cluster to give two spectroscopically distinct forms RI and RII which are reversibly interconvertible with a pKa approximately 7.5. The higher-pH form, RII, has a high affinity for ferrous ion and converts readily to a [4Fe-4S]1+ cluster, scavenging iron from the medium. The presence of the iron chelator EDTA inhibits this conversion.
摘要
通过低温磁圆二色性和电子顺磁共振光谱对来自褐球固氮菌的铁氧化还原蛋白进行了研究。需氧条件下分离得到的铁氧化还原蛋白含有一个[3Fe-4S]簇和一个[4Fe-4S]簇。在乙二醇存在下用连二亚硫酸盐进行厌氧处理会将[3Fe-4S]簇还原，产生两种光谱上不同的形式RI和RII，它们可在pKa约为7.5时可逆地相互转化。较高pH值的形式RII对亚铁离子具有高亲和力，并容易转化为[4Fe-4S]1+簇，从培养基中清除铁。铁螯合剂EDTA的存在会抑制这种转化。
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