Buxton B A, Schultz R D
Can J Comp Med. 1984 Oct;48(4):365-9.
Peripheral blood mononuclear cells were obtained from 13 bovine leukosis virus infected cattle and inoculated subcutaneously into 29 recipient adult steers to determine (a) the number of mononuclear cells (equivalent amount of blood) necessary to cause infection and (b) factors influencing infectivity of mononuclear cells from bovine leukosis virus-infected animals. A total of 55 inoculations were made. Inoculation of 1 X 10(4), 2 X 10(4) and 5 X 10(4) mononuclear cells caused seroconversion in 12%, 57% and 62% of steers, respectively. No infections occurred with 1 X 10(3) or 2 X 10(3) mononuclear cells. Cattle infected for longer than 24 months and those animals greater than three years of age were more likely to cause infection with 1 to 5 X 10(4) mononuclear cells than were cattle infected for less than 24 months or animals less than three years of age. Lymphocytes from cattle with persistent lymphocytosis caused more infections when 1 X 10(4) or 2 X 10(4) mononuclear cells were inoculated, than did lymphocytes from nonpersistent lymphocytosis cattle; however, both groups were equally infectious when 5 X 10(4) mononuclear cells were inoculated. No differences were found in infectivity of experimentally vs naturally exposed animals.
从13头感染牛白血病病毒的牛身上获取外周血单个核细胞，并将其皮下接种到29头成年受体公牛体内，以确定(a)引起感染所需的单个核细胞数量（等量血液），以及(b)影响来自感染牛白血病病毒动物的单个核细胞感染性的因素。总共进行了55次接种。接种1×10⁴、2×10⁴和5×10⁴个单个核细胞后，分别有12%、57%和62%的公牛出现血清转化。接种1×10³或2×10³个单个核细胞未发生感染。感染超过24个月的牛和年龄大于3岁的动物，与感染少于24个月的牛或年龄小于3岁的动物相比，用1至5×10⁴个单个核细胞更易引起感染。接种1×10⁴或2×10⁴个单个核细胞时，患有持续性淋巴细胞增多症的牛的淋巴细胞比非持续性淋巴细胞增多症牛的淋巴细胞引起更多感染；然而，接种5×10⁴个单个核细胞时，两组的感染性相同。在实验暴露动物与自然暴露动物的感染性方面未发现差异。
