Buxton B A, Schultz R D, Collins W E
Am J Vet Res. 1982 Aug;43(8):1458-9.
Bovine leukosis virus (BLV) was transmitted to sheep in a simulated mechanical transmission experiment, using the following species of mosquitoes; Anopheles freeborni, A stephensi, A quadrimaculatus, and A albimanus. Mosquitoes were fed on blood taken from a BLV-infected cow with persistent lymphocytosis. Mouthparts and heads of mosquitoes were removed immediately after feeding, placed in RPMI 1640 medium, and inoculated subcutaneously into sheep. Nine sheep were inoculated with mouthparts and heads from 37 to 122 mosquitoes. Infection was determined serologically. Three monthly serum samples were collected from the sheep and were tested for the presence of antibodies to BLV, using the agar-gel immunodiffusion (AGID) test. Sera that were negative by AGID at 3 months were tested by radioimmunoassay. Results from radioimmunoassay agreed with those obtained by AGID. Four of the 9 sheep developed antibody to BLV. Sheep that seroconverted were inoculated with mouthparts and heads from as few as 54 mosquitoes.
在一项模拟机械传播实验中，使用以下几种蚊子将牛白血病病毒（BLV）传播给绵羊：弗里伯恩按蚊、斯氏按蚊、四斑按蚊和白跗按蚊。让蚊子吸食感染了BLV且患有持续性淋巴细胞增多症的奶牛的血液。喂食后立即移除蚊子的口器和头部，将其置于RPMI 1640培养基中，并皮下接种到绵羊体内。用37至122只蚊子的口器和头部接种了9只绵羊。通过血清学方法确定感染情况。每月从绵羊身上采集三次血清样本，并使用琼脂凝胶免疫扩散（AGID）试验检测是否存在抗BLV抗体。在3个月时AGID检测为阴性的血清通过放射免疫测定法进行检测。放射免疫测定法的结果与AGID法获得的结果一致。9只绵羊中有4只产生了抗BLV抗体。血清转化的绵羊接种了少至54只蚊子的口器和头部。
