Hanna Z, Fregeau C, Préfontaine G, Brousseau R
Gene. 1984 Oct;30(1-3):247-50. doi: 10.1016/0378-1119(84)90128-8.
A family of universal expression vectors based on the pUC8 and pUC9 plasmids has been constructed. These vectors cover all three possible reading frames in both directions, allowing any synthetic DNA, genomic DNA or cDNA to be expressed under control of the lac promoter. The four new vectors retain the useful features of the pUC plasmids, including the blue to white color change on X-gal plates indicating the presence of an insert. This family of expression vectors is expected to be quite useful in allowing direct immunological screening of cDNA or genomic DNA banks.
基于pUC8和pUC9质粒构建了一个通用表达载体家族。这些载体涵盖了两个方向上所有三种可能的阅读框，使得任何合成DNA、基因组DNA或cDNA都能在lac启动子的控制下表达。这四个新载体保留了pUC质粒的有用特性，包括在X-gal平板上由蓝变白的颜色变化，表明插入片段的存在。预计这个表达载体家族在对cDNA或基因组DNA文库进行直接免疫筛选方面会非常有用。
