Svensson L
J Gen Virol. 1984 Dec;65 ( Pt 12):2183-90. doi: 10.1099/0022-1317-65-12-2183.
An outer capsid glycoprotein of human rotavirus (serotype 1) was localized and characterized by use of lectins, electron microscopy and a modified Western blotting technique. Lectins with specificity for fucose, galactose, glucose and mannose were mixed with purified single- or double-shelled human rotavirus. Aggregates observed by electron microscopy were obtained with double-shelled virus and concanavalin A, the only tested lectin with mannose specificity. By SDS-polyacrylamide gel electrophoretic analysis nine structural polypeptides could be identified. Five of these polypeptides were components of the inner capsid (VP1, VP2, VP3, VP4, VP6) and four components of the outer capsid (VP5, VP7, VP8, VP9). When using a modified Western blotting technique employed for glycoprotein detection, only VP7 was found to be glycosylated. This glycoprotein could be identified in rotavirus from human stools as well as from cell cultures. Heterogeneity in molecular weight of VP7 was observed in different isolates. An unexpected heterogeneity of VP7 was seen within a human rotavirus strain. An unplaqued stock of virus was found to exhibit two distinguishable glycoprotein bands (VP7, VP7a). After 10 passages in MA-104 cells the same strain was found to exhibit only one glycoprotein band.
利用凝集素、电子显微镜和改良的蛋白质印迹技术对人轮状病毒（血清型1）的一种外衣壳糖蛋白进行了定位和特性分析。将对岩藻糖、半乳糖、葡萄糖和甘露糖具有特异性的凝集素与纯化的单壳或双壳人轮状病毒混合。通过电子显微镜观察到的凝集物是用双壳病毒和伴刀豆球蛋白A获得的，伴刀豆球蛋白A是唯一经测试的对甘露糖具有特异性的凝集素。通过SDS-聚丙烯酰胺凝胶电泳分析可以鉴定出9种结构多肽。其中5种多肽是内衣壳的成分（VP1、VP2、VP3、VP4、VP6），4种是外衣壳的成分（VP5、VP7、VP8、VP9）。当使用用于糖蛋白检测的改良蛋白质印迹技术时，发现只有VP7被糖基化。这种糖蛋白可以在来自人粪便以及细胞培养物的轮状病毒中鉴定出来。在不同分离株中观察到VP7分子量的异质性。在一株人轮状病毒株中发现了意想不到的VP7异质性。发现一份未形成空斑的病毒储备表现出两条可区分的糖蛋白条带（VP7、VP7a）。在MA-104细胞中传代10次后，发现同一株病毒只表现出一条糖蛋白条带。
