• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

人Ⅲ型前胶原的分子克隆及羧基前肽分析

Molecular cloning and carboxyl-propeptide analysis of human type III procollagen.

作者信息

Loidl H R, Brinker J M, May M, Pihlajaniemi T, Morrow S, Rosenbloom J, Myers J C

出版信息

Nucleic Acids Res. 1984 Dec 21;12(24):9383-94. doi: 10.1093/nar/12.24.9383.

DOI:10.1093/nar/12.24.9383
PMID:6096827
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320468/
Abstract

Two human cDNA libraries prepared from normal fibroblast (GM3348) and rhabdomyosarcoma (CCL136) mRNAs were screened under cross hybridization conditions with a genomic fragment coding for exons 2 and 3 of the avian type III procollagen COOH-propeptide (Yamada, Y., Mudryj, M., Sullivan, M. and deCrombrugghe, B. (1983) J. Biol. Chem. 258, 2758-2761). One cDNA clone containing a 1.12 kb insert was isolated from the CCL136 library and later used to identify a GM3348 derived clone with a 2.4 kb insert. Comparison of the human and avian type III C-terminal propeptides revealed much more divergence in the first 54 amino acids following the terminal cysteine of the triple helical region than is present in the alpha 1(I) and alpha 2(I) procollagen chains of these species. Analysis of poly (A+) RNA from normal human fibroblast and tumor cell lines showed that they differed greatly in the relative amounts of alpha 1(I), alpha 2(I), and alpha 1(III) mRNAs. Furthermore, as we previously reported for the alpha 1(I) and alpha 2(I) transcripts, multiple mRNAs also hybridize to the cloned alpha 1(III) DNA.

摘要

用编码禽Ⅲ型前胶原COOH-前肽外显子2和3的基因组片段，在交叉杂交条件下筛选了从正常人成纤维细胞（GM3348）和横纹肌肉瘤（CCL136）mRNA制备的两个人cDNA文库。从CCL136文库中分离出一个含1.12 kb插入片段的cDNA克隆，随后用于鉴定一个含2.4 kb插入片段的GM3348来源的克隆。人与禽Ⅲ型C端前肽的比较显示，在三螺旋区末端半胱氨酸后的前54个氨基酸中，其差异比这些物种的α1（I）和α2（I）前胶原链中的差异大得多。对正常人成纤维细胞和肿瘤细胞系的聚（A+）RNA分析表明，它们在α1（I）、α2（I）和α1（III）mRNA的相对含量上有很大差异。此外，正如我们先前报道的α1（I）和α2（I）转录本一样，多个mRNA也与克隆的α1（III）DNA杂交。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea2a/320468/a92890367815/nar00342-0165-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea2a/320468/a92890367815/nar00342-0165-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea2a/320468/a92890367815/nar00342-0165-a.jpg

相似文献

1
Molecular cloning and carboxyl-propeptide analysis of human type III procollagen.人Ⅲ型前胶原的分子克隆及羧基前肽分析
Nucleic Acids Res. 1984 Dec 21;12(24):9383-94. doi: 10.1093/nar/12.24.9383.
2
Differential expression of a cysteine-rich domain in the amino-terminal propeptide of type II (cartilage) procollagen by alternative splicing of mRNA.通过mRNA的可变剪接，II型（软骨）前胶原氨基末端前肽中富含半胱氨酸结构域的差异表达。
J Biol Chem. 1990 Jun 25;265(18):10334-9.
3
Complete primary structure of the human alpha 2 type V procollagen COOH-terminal propeptide.人V型原胶原α2链羧基末端前肽的完整一级结构
J Biol Chem. 1985 Sep 15;260(20):11216-22.
4
Drosophila basement membrane procollagen alpha 1(IV). II. Complete cDNA sequence, genomic structure, and general implications for supramolecular assemblies.果蝇基底膜原胶原α1（IV）。II。完整的cDNA序列、基因组结构以及对超分子组装的一般意义。
J Biol Chem. 1988 Dec 5;263(34):18328-37.
5
Isolation and partial characterization of genomic clones coding for a human pro-alpha 1 (II) collagen chain and demonstration of restriction fragment length polymorphism at the 3' end of the gene.编码人原α1（II）胶原链的基因组克隆的分离及部分特性分析，以及该基因3'端限制性片段长度多态性的证明。
Biochemistry. 1985 Nov 5;24(23):6343-8. doi: 10.1021/bi00344a004.
6
Structure and sequence of the chicken type II procollagen gene. Characterization of the region encoding the carboxyl-terminal telopeptide and propeptide.鸡Ⅱ型原胶原基因的结构与序列。编码羧基末端端肽和前肽区域的特征分析。
J Biol Chem. 1984 Jun 25;259(12):7826-34.
7
Restricted homology between human alpha 1 type IV and other procollagen chains.
Proc Natl Acad Sci U S A. 1985 Jun;82(11):3649-53. doi: 10.1073/pnas.82.11.3649.
8
Partial covalent structure of the human alpha 2 type V collagen chain.人α2 V型胶原链的部分共价结构
J Biol Chem. 1985 May 10;260(9):5533-41.
9
Isolation and characterization of genomic clones corresponding to the human type II procollagen gene.与人类II型前胶原基因对应的基因组克隆的分离与鉴定。
Nucleic Acids Res. 1984 Jan 25;12(2):1025-38. doi: 10.1093/nar/12.2.1025.
10
Cloning a cDNA for the pro-alpha 2 chain of human type I collagen.克隆人I型胶原α2前体链的cDNA。
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3516-20. doi: 10.1073/pnas.78.6.3516.

引用本文的文献

1
Type III collagen (COL3A1): Gene and protein structure, tissue distribution, and associated diseases.III 型胶原（COL3A1）：基因和蛋白质结构、组织分布及相关疾病。
Gene. 2019 Jul 30;707:151-171. doi: 10.1016/j.gene.2019.05.003. Epub 2019 May 7.
2
Localization of precursor proteins and mRNA of type I and III collagens in usual interstitial pneumonia and sarcoidosis.I型和III型胶原蛋白前体蛋白及mRNA在寻常型间质性肺炎和结节病中的定位
J Mol Histol. 2005 Sep;36(6-7):437-46. doi: 10.1007/s10735-006-9018-9. Epub 2006 Mar 7.
3
Heparin/endothelial cell growth supplement regulates matrix gene expression and prolongs life span of vascular smooth muscle cells through modulation of interleukin-1.

本文引用的文献

1
Human cartilage collagens. Comparison of cartilage collagens with human type V collagen.人类软骨胶原蛋白。软骨胶原蛋白与人类V型胶原蛋白的比较。
J Biol Chem. 1982 Jul 10;257(13):7852-6.
2
Covalent structure of collagen: amino acid sequence of alpha 1(III)-CB9 from type III collagen of human liver.胶原蛋白的共价结构：来自人肝脏III型胶原蛋白的α1(III)-CB9的氨基酸序列。
Biochemistry. 1981 Apr 28;20(9):2621-7. doi: 10.1021/bi00512a040.
3
Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.通过在大肠杆菌中进行DNA融合和克隆分析基因控制信号。
肝素/内皮细胞生长补充剂通过调节白细胞介素-1来调节基质基因表达并延长血管平滑肌细胞的寿命。
In Vitro Cell Dev Biol Anim. 1999 Nov-Dec;35(10):647-54. doi: 10.1007/s11626-999-0105-6.
4
New lethal disease involving type I and III collagen defect resembling geroderma osteodysplastica, De Barsy syndrome, and Ehlers-Danlos syndrome IV.一种新的致死性疾病，涉及I型和III型胶原蛋白缺陷，类似于骨皮肤发育不全、德巴尔西综合征和埃勒斯-当洛综合征IV型。
J Med Genet. 1998 Jun;35(6):513-8. doi: 10.1136/jmg.35.6.513.
5
Expression of mRNAs for type I and type III procollagens in serous ovarian cystadenomas and cystadenocarcinomas.浆液性卵巢囊腺瘤和囊腺癌中I型和III型前胶原mRNA的表达
Am J Pathol. 1996 Feb;148(2):539-48.
6
Sequencing of cDNA from 50 unrelated patients reveals that mutations in the triple-helical domain of type III procollagen are an infrequent cause of aortic aneurysms.对50名无亲缘关系患者的cDNA进行测序后发现，III型前胶原三螺旋结构域的突变是主动脉瘤的罕见病因。
J Clin Invest. 1993 Jun;91(6):2539-45. doi: 10.1172/JCI116490.
7
Collagen genes and inherited connective tissue disease.胶原蛋白基因与遗传性结缔组织疾病
Biochem J. 1985 Jul 15;229(2):287-303. doi: 10.1042/bj2290287.
8
Human alpha 1(III) and alpha 2(V) procollagen genes are located on the long arm of chromosome 2.人类α1（III）和α2（V）前胶原基因位于2号染色体长臂上。
Proc Natl Acad Sci U S A. 1985 May;82(10):3385-9. doi: 10.1073/pnas.82.10.3385.
9
Co-ordinate increase in the expression of type I and type III collagen genes in progressive systemic sclerosis fibroblasts.进行性系统性硬化症成纤维细胞中I型和III型胶原基因表达的协同增加。
Biochem J. 1986 Aug 1;237(3):837-43. doi: 10.1042/bj2370837.
10
Localization of the human procollagen alpha 1(IV) gene to chromosome 13q34 by in situ hybridization.通过原位杂交将人类原胶原蛋白α1(IV)基因定位于染色体13q34。
Am J Hum Genet. 1986 Jan;38(1):38-44.
J Mol Biol. 1980 Apr;138(2):179-207. doi: 10.1016/0022-2836(80)90283-1.
4
Sequence determination and analysis of the 3' region of chicken pro-alpha 1(I) and pro-alpha 2(I) collagen messenger ribonucleic acids including the carboxy-terminal propeptide sequences.鸡原α1（I）和原α2（I）型胶原信使核糖核酸3'区域的序列测定与分析，包括羧基末端前肽序列。
Biochemistry. 1981 Feb 17;20(4):996-1006. doi: 10.1021/bi00507a054.
5
Identification and partial characterization of three low-molecular-weight collagenous polypeptides synthesized by chondrocytes cultured within collagen gels in the absence and in the presence of fibronectin.在不存在和存在纤连蛋白的情况下，对在胶原凝胶中培养的软骨细胞合成的三种低分子量胶原多肽进行鉴定和部分特性分析。
Biochem J. 1983 May 1;211(2):417-26. doi: 10.1042/bj2110417.
6
A conserved nucleotide sequence, coding for a segment of the C-propeptide, is found at the same location in different collagen genes.在不同的胶原蛋白基因中，编码C-前肽片段的保守核苷酸序列位于相同位置。
Nucleic Acids Res. 1983 May 11;11(9):2733-44. doi: 10.1093/nar/11.9.2733.
7
Further characterization of the three polypeptide chains of bovine and human short-chain collagen (intima collagen).牛和人短链胶原蛋白（内膜胶原蛋白）三条多肽链的进一步特性分析。
Eur J Biochem. 1983 Jun 1;133(1):39-46. doi: 10.1111/j.1432-1033.1983.tb07427.x.
8
Posttranslational modifications in the biosynthesis of type IV collagen by a human tumor cell line.人肿瘤细胞系在IV型胶原蛋白生物合成中的翻译后修饰
Biochemistry. 1981 Dec 22;20(26):7409-15. doi: 10.1021/bi00529a014.
9
Nucleotide sequences of complementary deoxyribonucleic acids for the pro alpha 1 chain of human type I procollagen. Statistical evaluation of structures that are conserved during evolution.人I型前胶原原α1链互补脱氧核糖核酸的核苷酸序列。对进化过程中保守结构的统计学评估。
Biochemistry. 1983 Oct 25;22(22):5213-23. doi: 10.1021/bi00291a023.
10
The mRNAs for the pro-alpha 1(I) and pro-alpha 2(I) chains of type I procollagen are translated at the same rate in normal human fibroblasts and in fibroblasts from two variants of osteogenesis imperfecta with altered steady state ratios of the two mRNAs.I型前胶原的α1（I）前体链和α2（I）前体链的信使核糖核酸（mRNA），在正常人成纤维细胞以及来自成骨不全两种变体的成纤维细胞中，以相同速率进行翻译，这两种变体的两种mRNA的稳态比率有所改变。
J Biol Chem. 1983 Dec 10;258(23):14385-9.