Selmer J C, Larsen F S, Hertz J B, Parton R
Acta Pathol Microbiol Immunol Scand C. 1984 Oct;92(5):279-84. doi: 10.1111/j.1699-0463.1984.tb00088.x.
Two monoclonal antibodies against filamentous haemagglutinin (F-HA) from Bordetella have been produced. In immunoblotting of a SDS-polyacrylamide gel electrophoresis (SDS-PAGE) of high ionic strength extracts of Bordetella pertussis, the two monoclonal antibodies both stained several high molecular weight bands. The two monoclonal antibodies were used for immuno-affinity column one-step purification of F-HA from high-ionic-strength extracts of Bordetella pertussis. In gradient SDS-PAGE, the eluted F-HA is present as a major double band with a molecular weight of approximately 240,000 daltons and several minor bands with molecular weights down to 90,000 daltons. The monoclonal antibodies were used in a monoclonal antibody catching enzyme linked immunosorbent assay (ELISA) for sensitive and specific detection of F-HA. This ELISA system proved valuable in optimizing the elution conditions for the monoclonal affinity columns.
已制备出两种针对博德特氏菌丝状血凝素（F-HA）的单克隆抗体。在对百日咳博德特氏菌高离子强度提取物进行的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）免疫印迹中，这两种单克隆抗体均使几条高分子量条带显色。这两种单克隆抗体用于从百日咳博德特氏菌高离子强度提取物中一步免疫亲和柱纯化F-HA。在梯度SDS-PAGE中，洗脱的F-HA呈现为一条主要的双链带，分子量约为240,000道尔顿，还有几条分子量低至90,000道尔顿的次要条带。这些单克隆抗体用于单克隆抗体捕获酶联免疫吸附测定（ELISA），以灵敏且特异地检测F-HA。该ELISA系统在优化单克隆亲和柱的洗脱条件方面被证明是有价值的。
