Barb C R, Kraeling R R, Rampacek G B, Pinkert C A
Biol Reprod. 1984 Dec;31(5):913-9. doi: 10.1095/biolreprod31.5.913.
The effect of prostaglandin F2 alpha (PGF2 alpha) on luteinizing hormone (LH) receptors, weight and progesterone content of corpora lutea (CL), and serum progesterone concentrations was studied in gilts. Fifteen gilts were hysterectomized between Days 9 to 11 of the estrous cycle. Twelve gilts were injected i.m. with 10 mg of PGF2 alpha and 3 with saline on Day 20. Ovaries were surgically removed from each of 3 gilts at 4, 8, 12 and 24 h following PGF2 alpha treatment and from the 3 control gilts 12 h following saline injection. Jugular blood samples for progesterone analysis were collected from all gilts at 0, 2 and 4 h following treatment and at 8, 12 and 24 h for gilts from which ovaries were removed at 8, 12 and 24 h, respectively. Mean serum progesterone and CL progesterone concentrations decreased within 4 h after PGF2 alpha treatment (P less than 0.05) and remained low through 24 h after treatment. The number of unoccupied LH receptors decreased by 4 h (P less than 0.05) and this trend continued through 24 h. There were no differences in luteal weight or affinity of unoccupied LH receptors of luteal tissue at 4, 8 12 and 24 h after PGF2 alpha when compared to luteal tissue from controls. These data indicate that during PGF2 alpha-induced luteolysis in the pig, luteal progesterone, serum progesterone concentrations and the number of LH receptors decrease simultaneously.
研究了前列腺素F2α（PGF2α）对后备母猪黄体生成素（LH）受体、黄体（CL）重量和孕酮含量以及血清孕酮浓度的影响。在发情周期的第9至11天，对15头后备母猪进行子宫切除。在第20天，12头后备母猪肌肉注射10mg PGF2α，3头注射生理盐水。在PGF2α处理后4、8、12和24小时，从3头后备母猪中手术摘除卵巢，并在注射生理盐水12小时后从3头对照后备母猪中摘除卵巢。在处理后0、2和4小时，从所有后备母猪采集颈静脉血样进行孕酮分析；对于分别在8、12和24小时摘除卵巢的后备母猪，在8、12和24小时采集血样。PGF2α处理后4小时内，血清孕酮和CL孕酮的平均浓度下降（P<0.05），并在处理后24小时内一直保持在低水平。未占据的LH受体数量在4小时时减少（P<0.05），这一趋势持续到24小时。与对照组黄体组织相比，PGF2α处理后4、8、12和24小时，黄体重量或黄体组织中未占据的LH受体亲和力没有差异。这些数据表明，在PGF2α诱导的母猪黄体溶解过程中，黄体孕酮、血清孕酮浓度和LH受体数量同时下降。
