Justová V, Wildtová Z, Pacovský V
Endocrinol Exp. 1984 Dec;18(4):241-7.
Simplified competitive protein binding assay for determination of 1,25-dihydroxycholecalciferol, with chicken intestinal cytosol receptor as binding protein and tritiated 1,25-dihydroxycholecalciferol as a competitive radioligand was developed using the same outline procedure as for determination of the other biologically active metabolites of cholecalciferol. Binding characteristics (binding capacity and affinity) of a cytosol receptor were determined.
以鸡小肠胞质溶胶受体为结合蛋白，以氚标记的1,25 - 二羟胆钙化醇作为竞争性放射性配体，开发了一种用于测定1,25 - 二羟胆钙化醇的简化竞争性蛋白结合测定法，该方法采用与测定胆钙化醇其他生物活性代谢物相同的基本步骤。测定了胞质溶胶受体的结合特性（结合容量和亲和力）。
