从宋内志贺氏菌中分离出的一种微型质粒的特性分析。
Characterization of a mini plasmid isolated from Shigella sonnei.
作者信息
Sugiura S, Nakatani S, Mizukami Y, Hase T, Hirokawa H, Masamune Y
出版信息
J Biochem. 1984 Oct;96(4):1193-204. doi: 10.1093/oxfordjournals.jbchem.a134937.
Abstract
A multicopy plasmid, 2.1 kb in size, was isolated from Shigella sonnei and named pKYM. This plasmid is cryptic and isolated along with pKY-1, a ColE1-like plasmid. In this paper, we report the physical map of pKYM and some characters required for its multiplication. The replication of the plasmid DNA does not require DNA polymerase I but depends on protein(s) produced by itself. The plasmid is poorly mobilized by the F factor.
摘要
从宋内志贺氏菌中分离出一个大小为2.1 kb的多拷贝质粒，命名为pKYM。该质粒是隐蔽型的，与一个类ColE1质粒pKY-1一起被分离出来。在本文中，我们报道了pKYM的物理图谱及其复制所需的一些特性。质粒DNA的复制不需要DNA聚合酶I，但依赖于其自身产生的蛋白质。该质粒很难被F因子转移。
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