Kozubek S, Krasavin E A
Neoplasma. 1984;31(6):675-83.
Basic types of DNA damage produced by gamma-radiation in cells are reviewed. Different DNA injuries are related to various levels of DNA repair processes, established in the case of Escherichia coli cells. The role of the balance of repair enzyme activities is considered in connection with the induction of enzymatic DNA double-strand breaks (DSBs). The concept of "metastable sites" has been introduced. "Metastable sites" are formed from great nucleolytic gaps. They are measured as DSBs although they can be repaired as single-strand breaks (SSBs). A simple mathematical model of the inactivation of different mutants of E. coli cells has been constructed on the basis of available experimental data. Kinetic equations of the model have been solved and some parameters estimated for both sensitive and resistant mutants.
本文综述了γ射线在细胞中产生的DNA损伤的基本类型。不同的DNA损伤与大肠杆菌细胞中建立的不同水平的DNA修复过程有关。结合酶促DNA双链断裂（DSB）的诱导，探讨了修复酶活性平衡的作用。引入了“亚稳位点”的概念。“亚稳位点”由大的核酸酶切缺口形成。它们被测量为DSB，尽管它们可以作为单链断裂（SSB）进行修复。基于现有实验数据构建了大肠杆菌细胞不同突变体失活的简单数学模型。求解了该模型的动力学方程，并估计了敏感和抗性突变体的一些参数。
