Kristensen S D, Arnfred T, Dyerberg J
Thromb Res. 1984 Nov 15;36(4):305-14. doi: 10.1016/0049-3848(84)90321-9.
The production of prostacyclin (PGI2)-like material in human umbilical veins perfused continuously at 37 degrees C with Hanks buffer solution with 1% human albumin (HBA) was studied by bioassay. Subsequent perfusion resulted in a time dependent significant decrease in production of PGI2-like material. After addition of 20 mumol/l arachidonic acid (HBA-AA) the production of PGI2-like material increased significantly. The production of PGI2-like material was significant greater when the vein was perfused with HBA-AA than when perfused with HBA with 20 mumol/l eicosapentaenoic acid (HBA-EPA). Examination of the HBA-EPA perfusate by thin layer chromatography showed that it contained a substance that comigrated with genuine 6-keto-PGF1 alpha and a substance that comigrated with delta 17-6-keto-PGF1 alpha. Finally, perfusion with HBA containing 10 mumol/l AA plus 10 mumol/l EPA resulted in a significant greater production of PGI2-like material than perfusion with HBA-AA alone. These results support the hypothesis that EPA has beneficial antithrombotic properties in human.
采用生物测定法研究了在37℃下用含1%人白蛋白（HBA）的汉克斯缓冲溶液连续灌注的人脐静脉中类前列环素（PGI2）样物质的产生情况。后续灌注导致类PGI2样物质的产生随时间显著减少。加入20μmol/L花生四烯酸（HBA - AA）后，类PGI2样物质的产生显著增加。当静脉用HBA - AA灌注时，类PGI2样物质的产生显著高于用含20μmol/L二十碳五烯酸（HBA - EPA）的HBA灌注时。用薄层色谱法检测HBA - EPA灌注液表明，它含有一种与真正的6 - 酮 - PGF1α共迁移的物质和一种与δ17 - 6 - 酮 - PGF1α共迁移的物质。最后，用含10μmol/L AA加10μmol/L EPA的HBA灌注比单独用HBA - AA灌注导致类PGI2样物质的产生显著增加。这些结果支持了EPA对人类具有有益抗血栓形成特性这一假说。
