Churchward G, Linder P, Caro L
Adv Exp Med Biol. 1984;179:209-14. doi: 10.1007/978-1-4684-8730-5_21.
We describe the mapping of several genetic loci involved in the replication of the pSC101 plasmid. These include the origin of replication and a short segment of DNA that encodes a pSC101 incompatibility function. This short segment lies within the origin region. Flanking the incompatibility segment are two loci, repA and repB, which are required for replication. The product of the repA locus is shown to be trans-acting.
我们描述了参与pSC101质粒复制的几个基因位点的定位。这些位点包括复制起点以及一段编码pSC101不相容性功能的短DNA片段。这段短片段位于起点区域内。在不相容性片段两侧是两个复制所需的位点，即repA和repB。repA位点的产物被证明具有反式作用。
