Whitfill C, Allen J, Gyles N R, Johnson Z, Thoma J A
Avian Dis. 1984 Oct-Dec;28(4):944-58.
Progressor (Pr) and regressor (R) chickens from the University of Arkansas lines were used as leukocyte donors. Peripheral blood leukocytes were stimulated with two mitogens, concanavalin A (Con A) and phytohemagglutinin-P (PHA-P). Both Pr and R leukocytes displayed the same Con A-stimulated dose dependency on blastogenesis. The regressor leukocytes were stimulated more than the progressor leukocytes at high concentrations of PHA-P. There did not appear to be any correlation between the response of individual chickens to mitogens and their ability to regress a Rous sarcoma. We concluded that the R-Rs-1 gene, which controls the animal's response to Rous sarcoma, and the Con A and PHA-P response genes are probably located at different loci in the chicken genome. Mitogen-induced blastogenesis does not appear to be a useful index to predict regression response in the chicken. Under our experimental conditions, regressor leukocytes showed a sensitization to sarcoma antigens, but the progressor leukocytes did not. The data suggest that the R-Rs-1 gene does not confer a generally enhanced immune status to the chicken but acts as an Ir response gene to augment the immune response to specific antigens.
来自阿肯色大学品系的进展型（Pr）和消退型（R）鸡被用作白细胞供体。外周血白细胞用两种有丝分裂原，即刀豆球蛋白A（Con A）和植物血凝素-P（PHA-P）进行刺激。Pr和R白细胞在Con A刺激下对细胞增殖显示出相同的剂量依赖性。在高浓度PHA-P刺激下，消退型白细胞比进展型白细胞受到的刺激更强。个体鸡对有丝分裂原的反应与其消退劳斯肉瘤的能力之间似乎没有任何相关性。我们得出结论，控制动物对劳斯肉瘤反应的R-Rs-1基因以及Con A和PHA-P反应基因可能位于鸡基因组的不同位点。有丝分裂原诱导的细胞增殖似乎不是预测鸡消退反应的有用指标。在我们的实验条件下，消退型白细胞对肉瘤抗原有致敏作用，而进展型白细胞则没有。数据表明，R-Rs-1基因并未赋予鸡普遍增强的免疫状态，而是作为一个Ir反应基因来增强对特定抗原的免疫反应。
