牛血清白蛋白：使用新型自旋标记对N端胃蛋白酶片段上脂肪酸结合位点的表征
Bovine serum albumin: characterization of a fatty acid binding site on the N-terminal peptic fragment using a new spin-label.
作者信息
Hsia J C, Wong L T, Tan C T, Er S S, Kharouba S, Balaskas E, Tinker D O, Feldhoff R C
出版信息
Biochemistry. 1984 Dec 4;23(25):5930-2. doi: 10.1021/bi00320a006.
A new spin-label, 4-(L-glutamo)-4'-[(1-oxy-2,2,5,5-tetramethyl-3L-pyrrolidinyl )amino]-3, 3'-dinitrodiphenyl sulfone, is shown to bind to one high-affinity binding site on bovine serum albumin (K = 5 X 10(4) M-1, n = 1). Analysis of the binding of the spin-label to the amino-terminal half (peptic fragment PB) and the carboxy-terminal half (peptic fragment PA) of BSA, and their complex (PA-PB), indicates that the spin-label binds to a long-chain fatty acid binding site located on PB. The usefulness of the novel specificity of the spin-label in characterizing this binding site is discussed.
一种新的自旋标记物，4-(L-谷氨酰胺基)-4'-[(1-氧代-2,2,5,5-四甲基-3-L-吡咯烷基)氨基]-3,3'-二硝基二苯砜，已被证明能与牛血清白蛋白上的一个高亲和力结合位点结合（K = 5×10⁴ M⁻¹，n = 1）。对自旋标记物与牛血清白蛋白氨基末端半段（胃蛋白酶片段PB）、羧基末端半段（胃蛋白酶片段PA）及其复合物（PA-PB）结合情况的分析表明，自旋标记物结合到位于PB上的一个长链脂肪酸结合位点。讨论了这种自旋标记物的新特异性在表征该结合位点方面的用途。
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